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There is evidence from these studies that treatment of Stage B1 dogs with pimobendan may cause (a) increased regurgitation of blood through the mitral valve, (b) deterioration of the chordae tendineae, and (c) enlargement of the left side of the heart

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Frozen hind paws were used for the estimation of lipid peroxides (malondialdehyde, MDA), nitric oxide (NO), reduced glutathione (GSH), TNF-alpha level and HO-1 messenger RNA (mRNA) expression